The announcement by trainer Doug O'Neill ends the Canadian-owned chestnut colt's bid to become the first Triple Crown winner in 34 years, according to CNN.
”It’s not tragic, but it’s a huge disappointment,” O’Neill said.
He planned to walk the horse in front of the media at an afternoon press conference to demonstrate that the animal looks fine, but has the beginning of a leg injury and cannot run the race.
When asked on the "Dan Patrick Show" if I'll Have Another has run his last race, O'Neill responded: "If I had to wager ... I would say yes."
Rumors of the scratch began floating on Twitter a few minutes before the official confirmation, when word spread that O’Neill was holding a press conference this afternoon.